Sen Zhang

2 months ago | nature.com | Zhe Yu |Sen Zhang |Ju Chen |Julius Bogomolovas |Sylvia M. Evans

Cardiac regeneration studies have been plagued by technical challenges in unequivocally identifying cardiomyocyte (CM) nuclei in cardiac sections, crucial for accurate identification of cycling CMs. The use of antibodies to sarcomeric proteins is error-prone, the CM specificity of common nuclear markers is controversial, and utilizing genetically modified mouse models poses risk of inducing unintended cardiac phenotypes. The application of RNAscope intronic probes overcomes the above shortcomings. Intronic probes label intronic RNAs within nuclei and can therefore be utilized as a method for nuclear localization. A Tnnt2 intronic RNAscope probe highly colocalized with Obscurin-H2B-GFP in adult mouse hearts, demonstrating CM specificity. Studies in embryos demonstrated that the Tnnt2 intronic RNAscope probe labeled CM nuclei that had undergone DNA replication, and remained closely associated with CM chromatin at all stages of mitosis, even with nuclear envelope breakdown. The efficiency, accuracy, and perdurance of the Tnnt2 intronic RNAscope probe even with nuclear envelope breakdown facilitated reliable investigation of dynamics of DNA synthesis and potential mitoses in CMs in both border and infarct zones after myocardial infarction (MI). Furthermore, we designed Myl2 and Myl4 intronic RNAscope probes, which labeled ventricular and atrial CM nuclei, respectively, and may help identify CM subtypes generated in vitro. Intronic RNA probes precisely identify cardiomyocyte nuclei or chromatin throughout the cell cycle during embryonic cardiomyocyte proliferation and precisely identify cardiomyocyte nuclei in adult heart, enabling cardiac regeneration studies.

Nov 26, 2024 | nature.com | Sen Zhang

AbstractThis paper proposes a control strategy for the variable boost characteristics of electronic hydraulic brake (EHB) systems based on the driving style in response to the nonlinear challenges faced by the EHB systems in intelligent driving under complex personalized requirements. Initially, the working principle of the active braking of the EHB-booster was analyzed, and equivalent dynamic models and Karnopp friction models were established.

Nov 21, 2024 | onlinelibrary.wiley.com | Yuchang Li |Jing Li |Sen Zhang |Runxin Liang

1 Introduction Tick-borne encephalitis virus (TBEV) belongs to Orthoflavivirus genus, Flaviviridae family. The virus is a minus- strand RNA virus with a spherical particle and envelope protein on its surface. TBEV is transmitted primarily through the bite of the infected tick, the peak period of tick activity is from April to November each year, so TBEV generally spread in spring [1]. After tick biting, the virus can replicate under the skin of the patient.

Nov 7, 2023 | mdpi.com | Sen Zhang |Yisheng Zhang |Guoxing Liu |Chen Wang

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