Si Tao Xu

4 weeks ago | advanced.onlinelibrary.wiley.com | Bingqiang He |Si Tao Xu |Mengdi Li |Hui Li

Cell Culture and Treatment Primary astrocytes and microglia were isolated from the spinal cord of newborn SD rats at 1–2 days after birth according to the previous description.[51] Briefly, the dissected cords were immersed into 0.01 M PBS containing 1% penicillin-streptomycin (Beyotime, Cat# C0222), followed by mincing with scissors and digestion with 0.25% trypsin (Gibco, Cat# 25200072) for 15 min at 37 °C.