Sing-Hoi Sze

May 29, 2023 | biorxiv.org | Yunye Zhu |Irina O. Vvedenskaya |Sing-Hoi Sze |Bryce E. Nickels

AbstractTranscription start site (TSS) selection is a key step in gene expression and occurs at many promoter positions over a wide range of efficiencies. Here, we develop a massively parallel reporter assay to quantitatively dissect contributions of promoter sequence, NTP substrate levels, and RNA polymerase II (Pol II) activity to TSS selection by "promoter scanning" in Saccharomyces cerevisiae (Pol II MAssively Systematic Transcript End Readout, "Pol II MASTER").

Apr 4, 2023 | biorxiv.org | Bingbing Duan |Chenxi Qiu |Sing-Hoi Sze |Craig Kaplan

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Feb 28, 2023 | biorxiv.org | Bingbing Duan |Chenxi Qiu |Sing-Hoi Sze |Craig Kaplan

Thank you for your interest in spreading the word about bioRxiv. NOTE: Your email address is requested solely to identify you as the sender of this article. Your Email * Your Name * Send To * Enter multiple addresses on separate lines or separate them with commas. Message Subject (Your Name) has forwarded a page to you from bioRxiv Message Body (Your Name) thought you would like to see this page from the bioRxiv website.

Feb 28, 2023 | biorxiv.org | Irina O. Vvedenskaya |Bryce E. Nickels |Yunye Zhu |Sing-Hoi Sze

AbstractTranscription start site (TSS) selection is a key step in gene expression and occurs at many promoter positions over a wide range of efficiencies. Here, we develop a massively parallel reporter assay to quantitatively dissect contributions of promoter sequence, NTP substrate levels, and RNA polymerase II (Pol II) activity to TSS selection by "promoter scanning" in Saccharomyces cerevisiae (Pol II MAssively Systematic Transcript End Readout, "Pol II MASTER").