Yunye Zhu

Jan 3, 2024 | nature.com | Yunye Zhu |Bryce E. Nickels

AbstractTranscription start site (TSS) selection is a key step in gene expression and occurs at many promoter positions over a wide range of efficiencies. Here we develop a massively parallel reporter assay to quantitatively dissect contributions of promoter sequence, nucleoside triphosphate substrate levels and RNA polymerase II (Pol II) activity to TSS selection by ‘promoter scanning’ in Saccharomyces cerevisiae (Pol II MAssively Systematic Transcript End Readout, ‘Pol II MASTER’).

May 29, 2023 | biorxiv.org | Yunye Zhu |Irina O. Vvedenskaya |Sing-Hoi Sze |Bryce E. Nickels

AbstractTranscription start site (TSS) selection is a key step in gene expression and occurs at many promoter positions over a wide range of efficiencies. Here, we develop a massively parallel reporter assay to quantitatively dissect contributions of promoter sequence, NTP substrate levels, and RNA polymerase II (Pol II) activity to TSS selection by "promoter scanning" in Saccharomyces cerevisiae (Pol II MAssively Systematic Transcript End Readout, "Pol II MASTER").

Feb 28, 2023 | biorxiv.org | Irina O. Vvedenskaya |Bryce E. Nickels |Yunye Zhu |Sing-Hoi Sze

AbstractTranscription start site (TSS) selection is a key step in gene expression and occurs at many promoter positions over a wide range of efficiencies. Here, we develop a massively parallel reporter assay to quantitatively dissect contributions of promoter sequence, NTP substrate levels, and RNA polymerase II (Pol II) activity to TSS selection by "promoter scanning" in Saccharomyces cerevisiae (Pol II MAssively Systematic Transcript End Readout, "Pol II MASTER").