Siqi Guo

Dec 10, 2024 | nature.com | Kejia Li |Shijie Chen |Keyun Wang |Yan Wang |Yuying Ye |Zheng Fang | +14 more

By using a limited-proteolysis strategy that employs a large amount of trypsin to generate peptides directly from native proteins, we found that ligand-induced protein local stability shifts can be sensitively detected on a proteome-wide scale. This enabled us to develop the peptide-centric local stability assay, a modification-free approach that achieves unprecedented sensitivity in proteome-wide target identification and binding-region determination. We demonstrate the broad applications of the peptide-centric local stability assay by investigating interactions across various biological contexts. The peptide-centric local stability assay (PELSA) can be used to determine ligand-binding targets and binding regions on a proteome-wide scale with high sensitivity.

Apr 9, 2024 | link.aps.org | Junda Li |Jincheng Yue |Siqi Guo |Ao Zhang

The successful synthesis of the carbon-boron clathrate SrB3C3 under high-pressure conditions of 50 GPa opens up a new possibility for exploring high-temperature superconductors at ambient pressure. Based on the first-principle calculation, we designed a class of LaH10-like clathrate compounds Fm3¯m−XB2C8 (X = K, Rb, Cs, Sr, Ba, Ga, In, Tl, Sn, Pb, and Bi) and investigate their physical properties and potential superconductivities.

Nov 18, 2023 | pubs.rsc.org | Siqi Guo |Jincheng Yue |Junda Li |Yanhui Liu

Novel room-temperature Full-Heusler Thermoelectric material Li2TlSb High lattice thermal conductivity stemming from the intrinsically ordered crystal and strong interatomic bonds tends to be seen as the bottleneck to achieving excellent thermoelectric properties in Full-Heusler (FH) semiconductors.