Spartak Zikrin

3 weeks ago | nature.com | Daniela A. García-Soriano |David Fange |Dvir Schirman |Marco Grillo |Spartak Zikrin |Michael Ratz | +2 more

AbstractOptical pooled screening is an important tool to study dynamic phenotypes for libraries of genetically engineered cells. However, the desired engineering often requires that the barcodes used for in situ genotyping are expressed from the chromosome. This has not previously been achieved in bacteria. Here we describe a method for in situ genotyping of libraries with genomic barcodes in Escherichia coli.