Michael Ratz

3 weeks ago | nature.com | Daniela A. García-Soriano |David Fange |Dvir Schirman |Marco Grillo |Spartak Zikrin |Michael Ratz | +2 more

AbstractOptical pooled screening is an important tool to study dynamic phenotypes for libraries of genetically engineered cells. However, the desired engineering often requires that the barcodes used for in situ genotyping are expressed from the chromosome. This has not previously been achieved in bacteria. Here we describe a method for in situ genotyping of libraries with genomic barcodes in Escherichia coli.

Apr 23, 2024 | nature.com | Luuk Harbers |Michele Simonetti |Constantin Diekmann |Enrico Berrino |Silvia Giordano |Anna Sapino | +7 more

AbstractSomatic copy number alterations (SCNAs) are pervasive in advanced human cancers, but their prevalence and spatial distribution in early-stage, localized tumors and their surrounding normal tissues are poorly characterized. Here, we perform multi-region, single-cell DNA sequencing to characterize the SCNA landscape across tumor-rich and normal tissue in two male patients with localized prostate cancer.