Stephen R. Quake

Dec 4, 2024 | biorxiv.org | Stephen R. Quake

AbstractThe Tabula Sapiens is a reference human cell atlas containing single cell transcriptomic data from more than two dozen organs and tissues. Here we report Tabula Sapiens 2.0 which includes data from nine new donors, doubles the number of cells in Tabula Sapiens, and adds four new tissues. This new data includes four donors with multiple organs contributed, thus providing a unique data set in which genetic background, age, and epigenetic effects are controlled for.

Nov 14, 2024 | cell.com | Stephen R. Quake

Main text So genocentric has modern biology become that we have forgotten that the real units of function and structure in an organism are cells and not genes.—Sydney Brenner (2002) Francis Crick devised the “Central Dogma” in trying to understand a particular problem: protein synthesis, and specifically the flow of information in protein synthesis.1 This great advance took place during the early days of the molecular biology revolution, when biologists were trying to understand the...

Jan 17, 2024 | nature.com | Shuai Wang |Wenfei Sun |Stephen R. Quake |Bryan Roth

AbstractThe assembly and specification of synapses in the brain is incompletely understood1,2,3. Latrophilin-3 (encoded by Adgrl3, also known as Lphn3)—a postsynaptic adhesion G-protein-coupled receptor—mediates synapse formation in the hippocampus4 but the mechanisms involved remain unclear. Here we show in mice that LPHN3 organizes synapses through a convergent dual-pathway mechanism: activation of Gαs signalling and recruitment of phase-separated postsynaptic protein scaffolds.

Oct 3, 2023 | biorxiv.org | Shuai Wang |Thomas C. Südhof |Wenfei Sun |Stephen R. Quake

AbstractThe assembly and specification of synapses in the brain is incompletely understood. Latrophilin-3, a postsynaptic adhesion-GPCR, mediates synapse formation in the hippocampus but the mechanisms involved remain unclear. Here we show that Latrophilin-3 organizes synapses by a novel dual-pathway mechanism: activation of GαS/cAMP-signaling and recruitment of phase-separated postsynaptic protein scaffolds.