
Stewart WC Masson
Articles
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Jul 26, 2023 |
elifesciences.org | Stewart WC Masson |Søren Anker Madsen |Kristen C. Cooke |Meg Potter
The following is the authors’ response to the original reviews. Trypsinisation of the tissue prior to mitochondrial isolation is based on previous work and a Nature Protocol (1, 2) which isolated mitochondria for skeletal muscle. The rationale is that it aids in mechanical homogenisation from highly fibrous tissues such as quadriceps muscle by digesting extracellular matrix proteins.
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