Kristen C. Cooke

2 months ago | biorxiv.org | Søren Anker Madsen |Kristen C. Cooke |Meg Potter |Harry Benjamin Cutler

AbstractLipids, in particular ceramides and diacylglycerols (DAGs), are implicated in insulin resistance, however their precise roles remain unclear. We leverage natural genetic variation to examine muscle lipids and systemic insulin resistance (IR) in 399 Diversity Outbred Australia mice. Adipose mass was associated with 55% of muscle lipids and IR, with DAGs as the only enriched lipid class.

Jul 26, 2023 | elifesciences.org | Stewart WC Masson |Søren Anker Madsen |Kristen C. Cooke |Meg Potter

The following is the authors’ response to the original reviews. Trypsinisation of the tissue prior to mitochondrial isolation is based on previous work and a Nature Protocol (1, 2) which isolated mitochondria for skeletal muscle. The rationale is that it aids in mechanical homogenisation from highly fibrous tissues such as quadriceps muscle by digesting extracellular matrix proteins.