Tansol Choi

Jan 15, 2025 | biorxiv.org | Lauretta A. Lacko |Tansol Choi |Neranjan de Silva |Ying Liu

Abstract3-dimensional (3D) imaging is a powerful tool for interrogation of intact tissues, but can suffer from poor resolution due to impediments such as high tissue autofluorescence that remains a significant challenge in imaging cleared samples, including human clinical specimens. We developed Atacama Clear (ATC), a 3D imaging technology that increases signal-to-noise ratios (SNRs) while simultaneously augmenting the capacity of tissue to be cleared.

Oct 7, 2023 | mdpi.com | Tansol Choi |George Maiti |Shukti Chakravarti

4. Materials and Methods 4.1. Data SourcesFASTA sequences of mouse TLR9 and lumican were obtained from UniProt with primary accession numbers Q9EQU3 and P51885, respectively. The Class C CpG ODN_2395 sequence (5′-TCGTCGTTTTCGGCGCGCGCCG-3′) was obtained from Invivogen (#tlrl-2395). We used the crystal structure of the mouse TLR9 extracellular domain (TLR9 ECD) not bound to a ligand “unliganded form” (PDB:3WPF) obtained from Protein Data Bank [50].

Aug 22, 2023 | biorxiv.org | Tansol Choi |George Maiti |Shukti Chakravarti

AbstractLumican is an extracellular matrix proteoglycan, known to regulate toll-like receptor (TLR) signaling in innate immune cells. In experimental settings, lumican suppresses TLR9 signaling by binding to, and sequestering its synthetic ligand, CpG-DNA, in non-signal permissive endosomes. However, the molecular details of lumican interactions with CpG-DNA are obscure.