Thierry Soussi

Jan 7, 2025 | nature.com | Julianne Funk |Pascal Hunold |Dimitrios-Ilias Balourdas |Marek Bartkuhn |Rajkumar Savai |Thierry Soussi | +2 more

AbstractThe mutational landscape of TP53, a tumor suppressor mutated in about half of all cancers, includes over 2,000 known missense mutations. To fully leverage TP53 mutation status for personalized medicine, a thorough understanding of the functional diversity of these mutations is essential. We conducted a deep mutational scan using saturation genome editing with CRISPR-mediated homology-directed repair to engineer 9,225 TP53 variants in cancer cells.