Thomas W Thorpe

Sep 4, 2024 | zenodo.org | Thomas W Thorpe

Published September 4, 2024 | Version v1 Software Open Terrestriality, the adaptation to life on land, is one of the key evolutionary transitions, occurring numerous times across the tree of life. Within Arthropoda, there have been several independent transitions: in hexapods, myriapods, arachnids and isopods. Isopoda is a morphologically diverse order within Crustacea, with species adapted to almost every environment on Earth.

Jul 21, 2023 | onlinelibrary.wiley.com | Jonathan Dennis |Nick Johnson |Thomas W Thorpe |Stephen M. Wallace

Graphical Abstract Introducing new abiotic chemistry into living cells is a grand challenge in the field of chemical biotechnology. Here, we show that phosphate and/or biogenic amines mediate the non-enzymatic α-methylenation of butyraldehyde under biocompatible reaction conditions and can be interfaced with butyraldehyde biosynthesis from D-glucose in the bacterium Escherichia coli. Abstract Small molecule organocatalysts are abundant in all living organisms.

Jul 21, 2023 | pericles.pericles-prod.literatumonline.com | Jonathan Dennis |Nick Johnson |Thomas W Thorpe |Stephen M. Wallace

Abstract Small molecule organocatalysts are abundant in all living organisms. However, their use as organocatalysts in cells has been underexplored. Herein, we report that organocatalytic aldol chemistry can be interfaced with living Escherichia coli to enable the α-methylenation of cellular aldehydes using biogenic amines such as L-Pro or phosphate.