Vienna Biocenter

Jan 20, 2025 | genomebiology.biomedcentral.com | Austrian Academy |Vienna Biocenter

TE sequences were synthesized and cloned in pUC57 by Genescript. TEs were subsequently cloned in pCAMBIA3300 using restriction enzymes and a T4 DNA ligase-based cloning strategy. Plants were transformed via Agrobacterium tumefaciens floral dip [72] and T1 plants selected on appropriate herbicide for selection. All experiments were conducted on A. thaliana grown in vitro for two weeks on ½ MS plates under short light-day conditions (8-h light/16-h dark photoperiod and 22 °C).

Feb 24, 2024 | academic.oup.com | Ma Usa |Vienna Biocenter

Genomic intervals are one of the most prevalent data structures in computational genome biology, and used to represent features ranging from genes, to DNA binding sites, to disease variants. Operations on genomic intervals provide a language for asking questions about relationships between features.

Dec 4, 2023 | aacrjournals.org | Vienna Biocenter |Human Technopole |UNSW Sydney |Vita-Salute San Raffaele

This content is only available via PDF. This open access article is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License .

Nov 16, 2023 | academic.oup.com | Vienna Biocenter

In living organisms, many RNA molecules are modified post-transcriptionally. This turns the widely known four-letter RNA alphabet ACGU into a much larger one with currently more than 300 known distinct modified bases. The roles for the majority of modified bases remain uncertain, but many are already well-known for their ability to influence the preferred structures that an RNA may adopt. In fact, tRNAs sometimes require certain modifications to fold into their cloverleaf shaped structure.

Oct 25, 2023 | academic.oup.com | Vienna Biocenter

DNA damage represents a challenge to the genomic integrity on a daily basis. To address this constant threat, the eukaryotic cell developed a system integrating multiple pathways that act synergistically to promote cell survival in response to DNA damage. Once DNA damage is sensed, a cell cycle arrest accompanied by DNA repair signalling is initiated. Impaired repair mechanisms have a severe impact on fitness of the organism, leading to the onset of diseases such as cancer (reviewed in (1)).