Wah Chiu

Dec 6, 2024 | cell.com | Yanyan Zhao |Michael Schmid |Wah Chiu

Keywordscryogenic electron tomography subtomogram averagingcost-benefit analysisnear-atomic resolutioncumulative electron doseice thicknesssubtomogram numberstilt angle rangeGet full text accessLog in, subscribe or purchase for full access. References1. Zhang, K. ∙ Pintilie, G.D. ∙ Li, S. ... Resolving individual atoms of protein complex by cryo-electron microscopyCell Res. 2020; 30:1136-11392. Cogan, D.P. ∙ Zhang, K. ∙ Li, X. ...

Dec 2, 2024 | nature.com | Yagoub Adam |Robert T. Batey |Eugene F. Baulin |Janusz M Bujnicki |Kristy A. Carpenter |Wah Chiu | +44 more

AbstractRNA-Puzzles is a collective endeavor dedicated to the advancement and improvement of RNA three-dimensional structure prediction. With agreement from structural biologists, RNA structures are predicted by modeling groups before publication of the experimental structures. We report a large-scale set of predictions by 18 groups for 23 RNA-Puzzles: 4 RNA elements, 2 Aptamers, 4 Viral elements, 5 Ribozymes and 8 Riboswitches.

Oct 15, 2024 | biorxiv.org | Yanyan Zhao |Michael Schmid |Wah Chiu

AbstractThe folding nucleus (FN) initiates protein folding and enables an efficient folding pathway. Here we directly visualize the tubulin FN consisting of a nonnative, partially assembled Rossmann fold, in the closed chamber of human chaperonin TRiC. Chaperonin TRiC interacts with non-natively folded secondary structural elements, stabilizing the nucleus for transition into its first native domain.

Jun 25, 2024 | nature.com | Wah Chiu

Jan 31, 2024 | biorxiv.org | Yanyan Zhao |Michael Schmid |Wah Chiu

AbstractCryogenic electron microscopy single particle reconstruction (cryoEM-SPR) has evolved into a routine approach for determining macromolecule structures to near-atomic resolution. Cryogenic electron tomography subtomogram averaging (cryoET-STA) towards similar resolution, in contrast, is still under active development. CryoET can capture the 3D snapshot of individual molecules by stage tilting, offering multiple angular views per particle than the single particle reconstruction approach.