Yanyan Zhao

Dec 6, 2024 | cell.com | Yanyan Zhao |Michael Schmid |Wah Chiu

Keywordscryogenic electron tomography subtomogram averagingcost-benefit analysisnear-atomic resolutioncumulative electron doseice thicknesssubtomogram numberstilt angle rangeGet full text accessLog in, subscribe or purchase for full access. References1. Zhang, K. ∙ Pintilie, G.D. ∙ Li, S. ... Resolving individual atoms of protein complex by cryo-electron microscopyCell Res. 2020; 30:1136-11392. Cogan, D.P. ∙ Zhang, K. ∙ Li, X. ...

Oct 15, 2024 | biorxiv.org | Yanyan Zhao |Michael Schmid |Wah Chiu

AbstractThe folding nucleus (FN) initiates protein folding and enables an efficient folding pathway. Here we directly visualize the tubulin FN consisting of a nonnative, partially assembled Rossmann fold, in the closed chamber of human chaperonin TRiC. Chaperonin TRiC interacts with non-natively folded secondary structural elements, stabilizing the nucleus for transition into its first native domain.

Jan 31, 2024 | biorxiv.org | Yanyan Zhao |Michael Schmid |Wah Chiu

AbstractCryogenic electron microscopy single particle reconstruction (cryoEM-SPR) has evolved into a routine approach for determining macromolecule structures to near-atomic resolution. Cryogenic electron tomography subtomogram averaging (cryoET-STA) towards similar resolution, in contrast, is still under active development. CryoET can capture the 3D snapshot of individual molecules by stage tilting, offering multiple angular views per particle than the single particle reconstruction approach.

Aug 22, 2023 | pubs.rsc.org | Yang Zhao |Yu Guo |Yanyan Zhao |Xueke Yu

Two-dimensional fullerene-based monolayer materials assembled by C80 and Sc3N@C80 Construction of two-dimensional (2D) materials using fullerenes as building blocks has attracted particular attention, primarily due to their ability to integrate desired functionalities into devices. However, realization of stable 2D phases in the polymerized fullerenes remains a grand challenge.