Wei-Sheng W. Sun

Feb 15, 2024 | biorxiv.org | Wei-Sheng W. Sun |Gabriel Torrens |Josy ter Beek |Felipe Cava

AbstractConjugative Type 4 Secretion Systems (T4SS) are a main driver for the spread of antibiotic resistance genes and virulence factors in bacteria. To deliver the DNA substrate to recipient cells, it must cross the cell envelopes of both donor and recipient mating bacteria. In the T4SS from the enterococcal conjugative plasmid pCF10, PrgK is known to be the active cell wall degrading enzyme. It has 3 predicted extracellular hydrolase domains, LytM, SLT and CHAP.