Wolfram Seifert-Davila

Aug 28, 2024 | biorxiv.org | Wolfram Seifert-Davila |Anastasiia Chaban |Florence Baudin |Mathias Girbig

AbstractTranscription of tRNA genes by RNA polymerase III requires the general transcription factor IIIC (TFIIIC), which recognizes intragenic A-box and B-box DNA motifs of type II gene promoters. However, the underlying mechanism has remained elusive, in part due to missing structural information for A-box recognition.

Jul 7, 2023 | science.org | Gerald Coulis |Katherine Mateos |Akira Yokoi |Wolfram Seifert-Davila

AbstractTranscription factor (TF) IIIC recruits RNA polymerase (Pol) III to most of its target genes. Recognition of intragenic A- and B-box motifs in transfer RNA (tRNA) genes by TFIIIC modules τA and τB is the first critical step for tRNA synthesis but is mechanistically poorly understood. Here, we report cryo–electron microscopy structures of the six-subunit human TFIIIC complex unbound and bound to a tRNA gene.