Gerald Coulis

Jul 7, 2023 | science.org | Gerald Coulis |Katherine Mateos |Akira Yokoi |Xiaoyu Wang

AbstractThe blood circulation is considered the only way for the orally administered nanoparticles to enter the central nervous systems (CNS), whereas non-blood route-mediated nanoparticle translocation between organs is poorly understood. Here, we show that peripheral nerve fibers act as direct conduits for silver nanomaterials (Ag NMs) translocation from the gut to the CNS in both mice and rhesus monkeys.

Jul 7, 2023 | science.org | Gerald Coulis |Katherine Mateos |Akira Yokoi |Wolfram Seifert-Davila

AbstractTranscription factor (TF) IIIC recruits RNA polymerase (Pol) III to most of its target genes. Recognition of intragenic A- and B-box motifs in transfer RNA (tRNA) genes by TFIIIC modules τA and τB is the first critical step for tRNA synthesis but is mechanistically poorly understood. Here, we report cryo–electron microscopy structures of the six-subunit human TFIIIC complex unbound and bound to a tRNA gene.

Jul 7, 2023 | science.org | Gerald Coulis |Katherine Mateos |Akira Yokoi

AbstractPrior knowledge facilitates our perception and goal-directed behaviors, particularly when sensory input is lacking or noisy. However, the neural mechanisms underlying the improvement in sensorimotor behavior by prior expectations remain unknown. In this study, we examine the neural activity in the middle temporal (MT) area of visual cortex while monkeys perform a smooth pursuit eye movement task with prior expectation of the visual target’s motion direction.

Jul 7, 2023 | science.org | Gerald Coulis |Katherine Mateos |Akira Yokoi |Chao Chen

AbstractAutoproteolysis has been discovered to play key roles in various biological processes, but functional autoproteolysis has been rarely reported for transmembrane signaling in prokaryotes.

Jul 7, 2023 | science.org | Gerald Coulis |Katherine Mateos |Akira Yokoi |Feng Yuan

AbstractMembrane curvature is essential to diverse cellular functions. While classically attributed to structured domains, recent work illustrates that intrinsically disordered proteins are also potent drivers of membrane bending. Specifically, repulsive interactions among disordered domains drive convex bending, while attractive interactions drive concave bending, creating membrane-bound, liquid-like condensates.