Xin Zeng

2 months ago | nature.com | Wenchang Qian |Penglei Jiang |Mingming Niu |Yujuan Fu |Deyu Huang |Dong Zhang | +12 more

DNA methylation is a significant component in proximal chromatin regulation and plays crucial roles in regulating gene expression and maintaining the repressive state of retrotransposon elements. However, accurate profiling of the proteomics which simultaneously identifies specific DNA sequences and their associated epigenetic modifications remains a challenge. Here, we report a strategy termed SelectID (selective profiling of epigenetic control at genome targets identified by dCas9), which introduces methylated DNA binding domain into dCas9-mediated proximity labeling system to enable in situ protein capture at repetitive elements with 5-methylcytosine (5mC) modifications. SelectID is demonstrated as feasible as dCas9-TurboID system at specific DNA methylation regions, such as the chromosome 9 satellite. Using SelectID, we successfully identify CHD4 as potential repressors of methylated long interspersed nuclear element-1 (LINE-1) retrotransposon through direct binding at the 5’ untranslated region (5’UTR) of young LINE-1 elements. Overall, our SelectID approach has opened up avenues for uncovering potential regulators of specific DNA regions with DNA methylation, which will greatly facilitate future studies on epigenetic regulation. The authors introduce SelectID, an approach combining CRISPR-guided targeting with methylation-sensitive labeling to identify proteins interacting with methylated repetitive sequences. They suggest that CHD4 directly binds young LINE-1 retrotransposons, suppressing their activity.

Jan 12, 2025 | advanced.onlinelibrary.wiley.com | Jun Yang |Xin Zeng |Zhen Meng |Hongfang Liu

Conflict of Interest The authors declare no conflict of interest.

Jan 6, 2025 | onlinelibrary.wiley.com | Xin Zeng |Tian Wu |Qing Xu |Yujia Yuan

Conflicts of Interest The authors declare no conflicts of interest. Supporting Information Filename Description liv16234-sup-0001-DataS1.docxWord 2007 document , 38.6 KB Data S1. liv16234-sup-0002-FiguresS1-S12.docxWord 2007 document , 7.1 MB Figure S1. Figure S2. Figure S3. Figure S4. Figure S5. Figure S6. Figure S7. Figure S8. Figure S9. Figure S10. Figure S11. Figure S12.

Nov 10, 2024 | mdpi.com | Haoyong Chen |Xin Zeng |Yanjin Zhu |Tengteng Jia

1. IntroductionWith the increasing proportion of renewable energy sources in the power system, wind and solar power poses challenges to the safe and economic operation of the system. The electricity sector is the primary source of carbon dioxide emissions in China, and promoting low-carbon operation of the power system is crucial for its sustainable development [1].

Oct 9, 2024 | onlinelibrary.wiley.com | Zhoujian Wang |Xin Zeng |Lishu Shao |Na Liu

Conflicts of Interest The authors declare no conflicts of interest. Supporting Information Filename Description pol20240714-sup-0001-Supinfo.docxWord 2007 document , 5.2 MB Data S1. References 1, , , et al., “A Critical Review on Advances in the Practices and Perspectives for the Treatment of Dye Industry Wastewater,” Bioengineered 12 (2021): 70–87.