Xudong Wu

Oct 17, 2024 | biorxiv.org | Dan Zhao |Xudong Wu |Tom A. Rapoport |Westlake Laboratory

AbstractMisfolded glycoproteins in the endoplasmic reticulum (ER) lumen are translocated into the cytosol and degraded by the proteasome, a conserved process called ER-associated protein degradation (ERAD). In S. cerevisiae, the glycan of these proteins is trimmed by the luminal mannosidase Mnl1 (Htm1) to generate a signal that triggers degradation. Curiously, Mnl1 is permanently associated with protein disulfide isomerase (Pdi1).

Sep 1, 2024 | nature.com | Xudong Wu

Dear Editor,Norepinephrine (NE), also known as noradrenaline, is an important neurotransmitter that plays crucial roles in the central nervous system, including the regulation of mood, memory, and alertness. It also functions as a key hormone in the whole body. In the brain, NE transporter (NET, encoded by the SLC6A2 gene), a Na+/Cl–-dependent transporter, reuptakes released NE at neuron synapses, thus maintaining NE homeostasis.

Jun 20, 2024 | cell.com | Xudong Wu |Westlake Laboratory

Cryo-electron microscopy (cryoEM) is a powerful tool for studying protein structure and dynamics, revealing mechanisms of biological processes and aiding drug discovery. However, small and unstable proteins (<50 kDa) of pharmacological importance remain challenging targets. Additionally, large complexes from endogenous sources are often scarce for cryoEM study.

Apr 25, 2024 | nature.com | CHEN CHEN |Xudong Wu

AbstractLysine lactylation (Kla) links metabolism and gene regulation and plays a key role in multiple biological processes. However, the regulatory mechanism and functional consequence of Kla remain to be explored. Here, we report that HBO1 functions as a lysine lactyltransferase to regulate transcription. We show that HBO1 catalyzes the addition of Kla in vitro and intracellularly, and E508 is a key site for the lactyltransferase activity of HBO1.

Oct 14, 2023 | nature.com | Xudong Wu |Tao Zeng |Ying Liu

AbstractThe hippocampus plays major roles in learning and memory, and its formation requires precise coordination of patterning, cell proliferation, differentiation, and migration. Here we removed the chromatin-association capability of KDM2B in the progenitors of developing dorsal telencephalon (Kdm2b∆CxxC) to discover that Kdm2b∆CxxC hippocampus, particularly the dentate gyrus, became drastically smaller with disorganized cellular components and structure.