Xue-Ni Hou

Sep 1, 2023 | biorxiv.org | Chang Zhao |Mei-xia Ruan |Xu Dong |Xue-Ni Hou

AbstractProtein folding may involve folding intermediates. Ubiquitin (Ub) is a 76-residue small protein essential in post-translational modification and cell signaling. Ub is also a model system of protein folding. Previous studies have indicated the involvement of a folding intermediate, as Ub C-terminal residues, including strand β5, only dock correctly at a later stage. The natively folded Ub undergoes conformational dynamics over a vast range of timescales.