Yan Cai

Feb 27, 2024 | dx.doi.org | Yan Cai |Qin Yang |Rijing Liao |Chenchen Chang

Tandem mass tags (TMT) are widely used in proteomics to simultaneously quantify multiple samples in a single experiment. The tags can be easily added to the primary amines of peptides/proteins through chemical reactions. In addition to amines, TMT reagents also partially react with the hydroxyl groups of serine, threonine, and tyrosine residues under alkaline conditions, which significantly compromises the analytical sensitivity and precision.

Feb 7, 2024 | onlinelibrary.wiley.com | Yan Cai |Sichuan Chengdu Sichuan |Yuan Liu |Cong Wang

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