Yi-Fei Li

Sep 28, 2024 | nature.com | Yi-Fei Li |Yu Chi Liu |Chong Chen |Lu Chen |Xiangfeng Wang |Yi Liu

AbstractRNA-binding proteins (RBPs) modulate all aspects of RNA metabolism, but a comprehensive picture of RBP expression across tissues is lacking. Here, we describe our development of the method we call HARD-AP that robustly retrieves RBPs and tightly associated RNA regulatory complexes from cultured cells and fresh tissues. We successfully use HARD-AP to establish a comprehensive atlas of RBPs across mouse primary organs.