Yifeng Hong

Oct 11, 2024 | biorxiv.org | Yifeng Hong |Hanjie Wang |Michelle Wang

AbstractDuring DNA replication, the replisome must rotate relative to the DNA substrate, generating supercoiling that must be partitioned in front of or behind the replisome. Supercoiling partitioned behind the replisome may intertwine (or braid) daughter DNA molecules and restrict chromosome segregation. Supercoiling partitioning and torsional resistance at the replisome should depend on the geometry of the two daughter DNA molecules, determined by their end separations.

Sep 27, 2024 | biorxiv.org | Yifeng Hong |Fan Ye |Xiang Gao |James Inman

AbstractThe angular optical trap (AOT) is a powerful technique for measuring the DNA topology and rotational mechanics of fundamental biological processes. Realizing the full potential of the AOT requires rapid torsional control of these processes. However, existing AOT quartz cylinders are limited in their ability to meet the high rotation rate requirement while minimizing laser-induced photodamage.