Yinan Zhu

May 16, 2024 | cell.com | Raquel Ordoñez |Weimin Zhang |Gwen Ellis |Yinan Zhu |André M. Ribeiro-dos-Santos |Ran Brosh | +5 more

Genomic context critically modulates regulatory function but is difficult to manipulate systematically. The murine insulin-like growth factor 2 (Igf2)/H19 locus is a paradigmatic model of enhancer selectivity, whereby CTCF occupancy at an imprinting control region directs downstream enhancers to activate either H19 or Igf2. We used synthetic regulatory genomics to repeatedly replace the native locus with 157-kb payloads, and we systematically dissected its architecture.