Yusuke Takeshima

Jan 14, 2025 | biorxiv.org | Kelvin Chen |Yusuke Takeshima |Katsuhiro Makino |Andre Luiz Zorzetto-Fernandes

AbstractThe development of single-cell RNA-sequencing assays has transformed our understanding of cellular and tissue heterogeneity, yielding significant insights into disease biology and its underlying mechanisms. In this work, we describe icCITE-seq (intracellular cellular indexing of transcriptomes and epitopes), a scalable method that simultaneously measures surface and intracellular protein levels alongside gene expression across thousands of cells.