Yusuke Takeshima

Jan 14, 2025 | biorxiv.org | Kelvin Chen |Yusuke Takeshima |Katsuhiro Makino |Andre Luiz Zorzetto-Fernandes

AbstractThe development of single-cell RNA-sequencing assays has transformed our understanding of cellular and tissue heterogeneity, yielding significant insights into disease biology and its underlying mechanisms. In this work, we describe icCITE-seq (intracellular cellular indexing of transcriptomes and epitopes), a scalable method that simultaneously measures surface and intracellular protein levels alongside gene expression across thousands of cells.

May 10, 2023 | biorxiv.org | Yoshiaki Yasumizu |Yusuke Takeshima |Daiki Takeuchi |Reo Morimoto

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Apr 24, 2023 | nature.com | Yuya Shirai |Yusuke Takeshima |Shuhei Sakakibara |Yuta Yamaguchi |James B. Wing |Daisuke Okuzaki | +1 more

AbstractMechanisms underpinning the dysfunctional immune response in severe acute respiratory syndrome coronavirus 2 infection are elusive. We analyzed single-cell transcriptomes and T and B cell receptors (BCR) of >895,000 peripheral blood mononuclear cells from 73 coronavirus disease 2019 (COVID-19) patients and 75 healthy controls of Japanese ancestry with host genetic data. COVID-19 patients showed a low fraction of nonclassical monocytes (ncMono).