Adam Round

Jul 5, 2024 | nature.com | Leonardo Monrroy |Alfredo Bellisario |Richard Bean |Johan Bielecki |Richard A. Kirian |Romain Letrun | +6 more

AbstractDetecting microsecond structural perturbations in biomolecules has wide relevance in biology, chemistry and medicine. Here we show how MHz repetition rates at X-ray free-electron lasers can be used to produce microsecond time-series of protein scattering with exceptionally low noise levels of 0.001%. We demonstrate the approach by examining Jɑ helix unfolding of a light-oxygen-voltage photosensory domain.

Feb 1, 2024 | nature.com | Gábor Bortel |Marcin Sikorski |Richard Bean |Johan Bielecki |Adam Round |Miklós Tegze | +1 more

AbstractX-ray Free Electron Lasers (XFEL) are cutting-edge pulsed x-ray sources, whose extraordinary pulse parameters promise to unlock unique applications. Several new methods have been developed at XFELs; however, no methods are known, which allow ab initio atomic level structure determination using only a single XFEL pulse. Here, we present experimental results, demonstrating the determination of the 3D atomic structure from data obtained during a single 25 fs XFEL pulse.