Aiwei Guo

Sep 25, 2023 | mdpi.com | Aiwei Guo |Lily Liu |Mengxue Hu |Peifu Wu

2. Materials and Methods 2.1. ReagentsThe immortalized bovine mammary gland epithelial cell line MAC-T cells, of 40 generations, were provided by the laboratory of the Chinese Academy of Sciences, Kunming Institute of Zoology. The high glucose Dulbecco’s modified Eagle’s medium (DMED, Gibco™ 11965092, 99%), fetal bovine serum (FBS, Gibco™ 10099-141, 98%), and penicillin-streptomycin (Gibco™ 15140122, concentrated: 100×) were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Aug 6, 2023 | researchgate.net | Lily Liu |Aiwei Guo |Qin Zhang

Context 1... approximately 10 12 ~ 10 14 bacteria/mL, with Firmicutes, Bacteroidetes, Actinobacteria, and Verrucomicrobia as the dominant phyla. Furthermore, the relative abundance of these bacterial phyla in the small intestine and hindgut is higher than that in the rumen (14,19) (as shown in Table 1). Numerous studies have highlighted distinct differences in the microbial communities found in the rumen, small intestine and hindgut of dairy cows (5,10,20). ...

Aug 6, 2023 | researchgate.net | Lily Liu |Aiwei Guo |Qin Zhang

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Aug 1, 2023 | researchgate.net | Lily Liu |Aiwei Guo |Qin Zhang

Liu et al. 10.3389/fvets.2023.1206346Frontiers in Veterinary Science02frontiersin.organd is a key factor enabling cows to convert low-quality feed into high-quality milk and meat. is intricate ecosystem consists of diverse microorganisms, including bacteria, protozoa, and fungi. Currently, bacteria are the most extensively studied within the cow gut microbiota.