Arnold J. Boersma

Apr 13, 2024 | cell.com | Arnold J. Boersma

Due to the high concentration of proteins, nucleic acids, and other macromolecules, the bacterial cytoplasm is typically described as a crowded environment. However, the extent to which each of these macromolecules individually affects the mobility of macromolecular complexes, and how this depends on growth conditions, is presently unclear. In this study, we sought to quantify the crowding experienced by an exogenous 40 nm fluorescent particle in the cytoplasm of E.

Sep 28, 2023 | pubs.rsc.org | Sarah Mersch |Sarah Bergman |Arnold J. Boersma |Erin D. Sheets

Two-Photon Excited-State Dynamics of mEGFP–Linker–mScarlet–I Crowding Biosensor in Controlled Environments Macromolecular crowding affects many cellular processes such as diffusion, biochemical reaction kinetics, protein-protein interactions, and protein folding. Mapping the heterogeneous, dynamics crowding in living cells or tissues requires genetically encoded, site-specific, crowding sensors that are compatible with quantitative, noninvasive fluorescence micro-spectroscopy.