Sarah Bergman

Sep 28, 2023 | pubs.rsc.org | Sarah Mersch |Sarah Bergman |Arnold J. Boersma |Erin D. Sheets

Two-Photon Excited-State Dynamics of mEGFP–Linker–mScarlet–I Crowding Biosensor in Controlled Environments Macromolecular crowding affects many cellular processes such as diffusion, biochemical reaction kinetics, protein-protein interactions, and protein folding. Mapping the heterogeneous, dynamics crowding in living cells or tissues requires genetically encoded, site-specific, crowding sensors that are compatible with quantitative, noninvasive fluorescence micro-spectroscopy.