Blake T. Riley

Feb 18, 2025 | biorxiv.org | Liliana Abarca Guerrero |Ali Ebrahim |Blake T. Riley |Sean Kim

AbstractProtein tyrosine phosphatases (PTPs) play pivotal roles in myriad cellular processes by counteracting protein tyrosine kinases. Striatal-enriched protein tyrosine phosphatase (STEP, PTPN5) regulates synaptic function and neuronal plasticity in the brain and is a therapeutic target for several neurological disorders. Here, we present three new crystal structures of STEP, each with unexpected features.

Aug 30, 2024 | biorxiv.org | Simeon D. Castle |Adrian Woolfson |Blake T. Riley |Gregory Linshiz

AbstractDNA polymerases are complex molecular machines able to replicate genetic material using a template-driven process. While the copying function of these enzymes is well established, their ability to perform untemplated DNA synthesis is less well characterized. Here, we explore the ability of DNA polymerases to synthesize DNA fragments in the absence of template.