Cerdanyola del Vallès

Aug 20, 2024 | genomebiology.biomedcentral.com | Cerdanyola del Vallès

We outline the experimental procedure for RNA and ribosome footprint sequencing of HepG2 cells. The HepG2 cell line was obtained from the laboratory of Dr. Salvatore Piscuoglio (DBM, Basel) and was cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 4.5 g/l glucose, 10% fetal calf serum, 4 mM L-glutamine, 1X NEAA, 50 U/ml penicillin and 50 µg/ml streptomycin at 5% \(CO_2\), \(37^{\circ }\textrm{C}\). Cells were passaged every 3–4 days.

Aug 8, 2024 | bmcpsychiatry.biomedcentral.com | Cerdanyola del Vallès |Universitat Pompeu Fabra

Correction Open access Published: 10 October 2024 BMC Psychiatry volume 24, Article number: 673 (2024) Cite this article The Original Article was published on 08 August 2024 Fuentes et al. BMC Psychiatry (2024) 24:551Following publication of the original article [1], the authors would like to correct the funding statement. The changes have been highlighted in bold typeface.

Jul 28, 2024 | alzres.biomedcentral.com | Cerdanyola del Vallès |Clinical Laboratories

The production of human recombinant ApoJ (rhApoJ) has been previously reported [18, 23, 24]. Briefly, transfected human embryonic kidney 293T cells (HEK293T) overexpressing rhApoJ were cultured in HYPERFlask systems (Corning Inc., Corning, NY, USA).

Jul 28, 2024 | link.springer.com | Cerdanyola del Vallès |Clinical Laboratories

AbstractCerebral amyloid angiopathy (CAA) is characterized by amyloid-β (Aβ) deposition in cerebral vessels, leading to lobar cerebral microbleeds (CMB) and intracerebral hemorrhages (ICH). Apolipoprotein J (ApoJ) is a multifunctional chaperone related to Aβ aggregation and clearance. Our study investigated the vascular impact of chronic recombinant human Apolipoprotein J (rhApoJ) treatment in a transgenic mouse model of β-amyloidosis with prominent CAA.

Jul 15, 2024 | onlinelibrary.wiley.com | Campus UAB |Cerdanyola del Vallès

2.1. Chemicals Ex situ Pd/TiO2 samples were prepared using P25-type TiO2 (Evonik Operations GmbH) as a support. Palladium atomic absorption standard solution (1 mg ml−1 Pd in 5% HCl, Sigma-Aldrich) was used as a metal source. Both the support and the metal source, in a ratio to obtain 2 wt% Pd/TiO2, were dissolved in 0.1 M solution of formic acid in Mili-Q water with a total volume of 100 ml inside a batch-type quartz reactor cell, which was permanently flushed with N2.