Choli Lee

May 9, 2024 | nature.com | Jean-Benoît Lalanne |Diego Calderon |Beth Martin |Choli Lee |Cole Trapnell

AbstractThe inability to scalably and precisely measure the activity of developmental cis-regulatory elements (CREs) in multicellular systems is a bottleneck in genomics. Here we develop a dual RNA cassette that decouples the detection and quantification tasks inherent to multiplex single-cell reporter assays. The resulting measurement of reporter expression is accurate over multiple orders of magnitude, with a precision approaching the limit set by Poisson counting noise.