Cole Trapnell

Jul 1, 2024 | nature.com | Cole Trapnell

AbstractSingle-cell and spatial molecular profiling assays have shown large gains in sensitivity, resolution and throughput. Applying these technologies to specimens from human and model organisms promises to comprehensively catalogue cell types, reveal their lineage origins in development and discern their contributions to disease pathogenesis.

May 9, 2024 | nature.com | Jean-Benoît Lalanne |Diego Calderon |Beth Martin |Choli Lee |Cole Trapnell

AbstractThe inability to scalably and precisely measure the activity of developmental cis-regulatory elements (CREs) in multicellular systems is a bottleneck in genomics. Here we develop a dual RNA cassette that decouples the detection and quantification tasks inherent to multiplex single-cell reporter assays. The resulting measurement of reporter expression is accurate over multiple orders of magnitude, with a precision approaching the limit set by Poisson counting noise.

Feb 14, 2024 | nature.com | Chengxiang Qiu |Beth Martin |Riza M. Daza |Megan Taylor |Olivia Fulton |Xinxian Deng | +6 more

AbstractThe house mouse (Mus musculus) is an exceptional model system, combining genetic tractability with close evolutionary affinity to humans1,2. Mouse gestation lasts only 3 weeks, during which the genome orchestrates the astonishing transformation of a single-cell zygote into a free-living pup composed of more than 500 million cells.