Beth Martin

May 9, 2024 | nature.com | Jean-Benoît Lalanne |Diego Calderon |Beth Martin |Choli Lee |Cole Trapnell

AbstractThe inability to scalably and precisely measure the activity of developmental cis-regulatory elements (CREs) in multicellular systems is a bottleneck in genomics. Here we develop a dual RNA cassette that decouples the detection and quantification tasks inherent to multiplex single-cell reporter assays. The resulting measurement of reporter expression is accurate over multiple orders of magnitude, with a precision approaching the limit set by Poisson counting noise.

Feb 14, 2024 | nature.com | Chengxiang Qiu |Beth Martin |Riza M. Daza |Megan Taylor |Olivia Fulton |Xinxian Deng | +6 more

AbstractThe house mouse (Mus musculus) is an exceptional model system, combining genetic tractability with close evolutionary affinity to humans1,2. Mouse gestation lasts only 3 weeks, during which the genome orchestrates the astonishing transformation of a single-cell zygote into a free-living pup composed of more than 500 million cells.

Apr 12, 2023 | biorxiv.org | Xiaoyi Li |Wei Chen |Beth Martin |Diego Calderon

New Results doi: https://doi.org/10.1101/2023.04.12.536587 AbstractPrime editing is a powerful means of introducing precise changes to specific locations in mammalian genomes. However, the widely varying efficiency of prime editing across target sites of interest has limited its adoption in the context of both basic research and clinical settings. Here, we set out to exhaustively characterize the impact of the cis-chromatin environment on prime editing efficiency.