Ciara Y. Campbell

Oct 10, 2023 | nature.com | Daniel H. Goldhill |Carol M. Sheppard |Ksenia Sukhova |Jonathan Brown |Ciara Y. Campbell |Salik Nazki | +6 more

AbstractChickens genetically resistant to avian influenza could prevent future outbreaks. In chickens, influenza A virus (IAV) relies on host protein ANP32A. Here we use CRISPR/Cas9 to generate homozygous gene edited (GE) chickens containing two ANP32A amino acid substitutions that prevent viral polymerase interaction. After IAV challenge, 9/10 edited chickens remain uninfected. Challenge with a higher dose, however, led to breakthrough infections.