Daniel Fleischhauer

May 3, 2024 | cell.com | Dimitrios Papadopoulos |Am Hubland |Stefanie Anh Ha |Daniel Fleischhauer

Highlights•The MYCN oncoprotein exists in mutually exclusive DNA- and RNA-bound states•The conserved MYCBoxI mediates MYCN RNA binding in vitro and in cells•MYCN facilitates RNA processing as part of the nuclear exosome targeting complex•MYCN RNA binding supports S phase progression and tumor cell stress resilienceSummaryThe MYCN oncoprotein binds active promoters in a heterodimer with its partner protein MAX.

Aug 17, 2023 | biorxiv.org | Dimitrios Papadopoulos |Leonie Uhl |Stefanie Anh Ha |Daniel Fleischhauer

AbstractThe MYCN oncoprotein broadly binds active promoters in a heterodimer with its partner protein MAX. MYCN also interacts with the nuclear exosome, a 3'-5' exoribonuclease complex, suggesting a function in RNA metabolism. Here we show that MYCN forms stable high molecular weight complexes with the exosome and multiple RNA-binding proteins. In cells, MYCN binds to thousands of intronic RNAs; recombinant MYCN directly binds RNA via a short, highly conserved sequence termed MYCBoxI.