Leonie Uhl

Dec 6, 2024 | biorxiv.org | Leonie Uhl |Timothy J. Russell |Amel Aziba |Sinah Loebbert

AbstractIn response to perturbed transcription elongation, the MYC oncoprotein multimerizes and undergoes a phase transition; the underlying mechanisms and their function are unknown. Here, we show that MYC re-localizes from its canonical location on DNA to RNA in response to the accumulation of intronic RNA. MYC binds RNA directly, which enhances its multimerization.

Sep 25, 2023 | nature.com | Leonie Uhl |Benedetto Daniele Giaimo |Tobias Friedrich |Elisenda Alsina-Sanchis |David Sprinzak |Carolin Mogler | +5 more

AbstractCachexia is a major cause of morbidity and mortality in individuals with cancer and is characterized by weight loss due to adipose and muscle tissue wasting. Hallmarks of white adipose tissue (WAT) remodeling, which often precedes weight loss, are impaired lipid storage, inflammation and eventually fibrosis. Tissue wasting occurs in response to tumor-secreted factors.

Aug 17, 2023 | biorxiv.org | Dimitrios Papadopoulos |Leonie Uhl |Stefanie Anh Ha |Daniel Fleischhauer

AbstractThe MYCN oncoprotein broadly binds active promoters in a heterodimer with its partner protein MAX. MYCN also interacts with the nuclear exosome, a 3'-5' exoribonuclease complex, suggesting a function in RNA metabolism. Here we show that MYCN forms stable high molecular weight complexes with the exosome and multiple RNA-binding proteins. In cells, MYCN binds to thousands of intronic RNAs; recombinant MYCN directly binds RNA via a short, highly conserved sequence termed MYCBoxI.