Dhrubajyoti Datta

2 weeks ago | pubs.rsc.org | Dhrubajyoti Datta |Jayanta Kundu |Patrick Miller |Mehreen Khan

Expanding the binding space of argonaute-2: incorporation of either E or Z isomers of 6′-vinylphosphonate at the 5′ end of the antisense strand improves RNAi activity† A phosphate or a phosphate mimic at the 5′ terminus of the antisense strand of a small interfering RNA (siRNA) is required for efficient loading into the RISC complex through the MID domain binding pocket of Ago2.