Dominika Dobosz

Jul 11, 2024 | cell.com | Ting-Yu Lin |Leon Kleemann |Dominika Dobosz |Michał Rawski

Highlights•Single-particle cryo-EM structures reveal how human PUS3 recognizes tRNAs•Two distinct interfaces in PUS3 are key for tRNA binding and positioning•PUS1- but not PUS3-dependent Ψ sites are found in RNAs other than tRNAs•Patient-derived mutations link PUS3’s tRNA modification activity to human diseaseSummaryPseudouridine (Ψ), the isomer of uridine, is ubiquitously found in RNA, including tRNA, rRNA, and mRNA.