Michał Rawski

Oct 3, 2024 | nature.com | Sebastian Pintscher |Rafał Pietras |Bohun Mielecki |Mateusz Szwalec |Anna Wójcik-Augustyn |Paulina Indyka | +3 more

AbstractA multi-subunit enzyme, cytochrome b6f (cytb6f), provides the crucial link between photosystems I and II in the photosynthetic membranes of higher plants, transferring electrons between plastoquinone (PQ) and plastocyanin. The atomic structure of cytb6f is known, but its detailed catalytic mechanism remains elusive.

Jul 11, 2024 | cell.com | Ting-Yu Lin |Leon Kleemann |Dominika Dobosz |Michał Rawski

Highlights•Single-particle cryo-EM structures reveal how human PUS3 recognizes tRNAs•Two distinct interfaces in PUS3 are key for tRNA binding and positioning•PUS1- but not PUS3-dependent Ψ sites are found in RNAs other than tRNAs•Patient-derived mutations link PUS3’s tRNA modification activity to human diseaseSummaryPseudouridine (Ψ), the isomer of uridine, is ubiquitously found in RNA, including tRNA, rRNA, and mRNA.

May 14, 2024 | nature.com | Marcin Jaciuk |Alexander Rau |Alexander Hammermeister |Michał Rawski |Paulina Indyka |Urszula Jankowska | +7 more

AbstracttRNA modifications affect ribosomal elongation speed and co-translational folding dynamics. The Elongator complex is responsible for introducing 5-carboxymethyl at wobble uridine bases (cm5U34) in eukaryotic tRNAs. However, the structure and function of human Elongator remain poorly understood. In this study, we present a series of cryo-EM structures of human ELP123 in complex with tRNA and cofactors at four different stages of the reaction.

Mar 27, 2023 | nature.com | Piotr Wilk |Artur Biela |Michał Rawski |Gert Bange |Sebastian Glatt

AbstractHypusination is a unique post-translational modification of the eukaryotic translation factor 5A (eIF5A) that is essential for overcoming ribosome stalling at polyproline sequence stretches. The initial step of hypusination, the formation of deoxyhypusine, is catalyzed by deoxyhypusine synthase (DHS), however, the molecular details of the DHS-mediated reaction remained elusive. Recently, patient-derived variants of DHS and eIF5A have been linked to rare neurodevelopmental disorders.