Elizabeth Zephyrine McDonough

Jul 19, 2023 | nature.com | Ellen Quardokus |Diane Saunders |Elizabeth Zephyrine McDonough |John Hickey |Christopher Werlein |Hua Tian | +17 more

AbstractMultiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods.

Jul 19, 2023 | nature.com | Yingnan Ju |Elizabeth Zephyrine McDonough |Katy Börner

AbstractMapping the human body at single cell resolution in three dimensions (3D) is important for understanding cellular interactions in context of tissue and organ organization. 2D spatial cell analysis in a single tissue section may be limited by cell numbers and histology. Here we show a workflow for 3D reconstruction of multiplexed sequential tissue sections: MATRICS-A (Multiplexed Image Three-D Reconstruction and Integrated Cell Spatial - Analysis).