Ellen Quardokus

Jul 19, 2023 | nature.com | Ellen Quardokus |Diane Saunders |Elizabeth Zephyrine McDonough |John Hickey |Christopher Werlein |Hua Tian | +17 more

AbstractMultiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods.

Jul 19, 2023 | nature.com | Rajeev Kumar Malhotra |Marc K. Halushka |Ellen Quardokus |Katy Börner

AbstractMore than 150 scientists from 17 consortia are collaborating on an international project to build a Human Reference Atlas, which maps all 37 trillion cells in the healthy adult human body. The initial release of this atlas provided hierarchical lists of the anatomical structures, cell types, and biomarkers in 11 organs.

Jul 19, 2023 | nature.com | Yashvardhan Jain |Leah L. Godwin |Yingnan Ju |Ellen Quardokus |Andreas Bueckle |Yiing Lin | +4 more

Data preparationFor the “HuBMAP – Hacking the Kidney” Kaggle competition, a dataset was compiled comprising 30 Periodic acid-Schiff (PAS) stain whole slide images (WSIs) with 7102 segmented renal glomeruli (see Supplementary Table 3 and “Methods”). To determine if algorithms generalize to other FTU types, a second dataset was compiled comprising seven colon hematoxylin and eosin (H&E) stain WSIs with 395 segmented colonic crypts (see Supplementary Table 4 and “Methods”).