Emigh Cortez

Dec 21, 2023 | zenodo.org | Emigh Cortez

Methods Rosetta modeling of hERG in open and closed states: We used the Rosetta structural modeling software (Bender et al., 2016;Leman et al., 2020) and the CryoEM structures of a putatively open-state hERG (PDB ID: 5VA2) (Wang and MacKinnon, 2017) and a closed-state EAG1 (PDB ID: 5K7L) (Whicher and MacKinnon, 2016) as templates (Figure 1). Each structure was passed through the cryo-EM density refinement protocol in Rosetta (Wang et al., 2016) (Supplement Script 1).

Jun 22, 2023 | frontiersin.org | Emigh Cortez |Mounir Tarek |Matthew Perry |Christopher Dempsey

1 IntroductionThe human ether-à-go-go-related gene (hERG) encodes voltage-gated potassium channel Kv11.1 that mediates the rapid repolarization phase during cardiac action potential (Sanguinetti and Tristani-Firouzi, 2006; Vandenberg et al., 2012). The hERG channel cycles between closed, open, and inactivated states in response to membrane voltage changes to tightly regulate K+ currents in the heart (Sanguinetti and Tristani-Firouzi, 2006; Vandenberg et al., 2012).