Emmanouela Petsolari

Mar 18, 2025 | biorxiv.org | Jim Horne |Elise Kaplan |Ben Jin |Emmanouela Petsolari

AbstractThe outer-membrane protein TolC from Escherichia coli belongs to an extensive superfamily whose members are found throughout the didermal, Gram-negative bacterial lineages. The protein serves as an activated exit duct in multi-drug efflux pumps and protein secretion machinery.

Dec 20, 2024 | science.org | Justin Joachim |Davide Maselli |Jurjan Aman |Pamela Swiatlowska |David Killock |Hiba Chaudhry | +10 more

MATERIALS AND METHODS Anti–p-ERM/anti–p-Dmoesin (Cell Signaling Technology, 3141), anti-GST (ChromoTek, 6G9), anti–p-Thr (Cell Signaling Technology, 9381), anti-GFP for Western blotting (ChromoTek, 3H9), anti-GFP for immunofluorescence detection of YFP-TNIK (Roche, 11814460001), anti-TNIK (Santa Cruz Biotechnology, sc-136103), anti-Actin (Sigma-Aldrich, A2228), anti–p-MLC2 Ser19 (Cell Signaling Technology, 3671), anti-Ezrin (Cell Signaling Technology, 3145), anti-moesin (Cell Signaling...