Erich G Chapman

2 weeks ago | biorxiv.org | Emily G Oldani |Adam Sachs |Erich G Chapman |Sunil Kumar

AbstractThe events that lead to protein misfolding diseases are not fully understood. Many proteins implicated in neurodegenerative diseases (e.g., TDP43) interact with nucleic acids, including RNA G-quadruplexes. In this work, we investigate whether RNA G-quadruplexes play a role in TDP43 condensation in biophysical and cellular models.

Apr 30, 2024 | biorxiv.org | Adam Sachs |Sunil Kumar |Emily G Oldani |Erich G Chapman

AbstractThe events that lead to protein misfolding diseases are poorly understood. Many proteins implicated in neurodegenerative diseases (e.g., TDP43) interact with nucleic acids, including RNA G-quadruplexes. In this work, we investigate whether RNA G-quadruplexes play a role in TDP43 aggregation in biophysical and cellular models. We find that G-quadruplexes modulate TDP43 aggregation in a biophysical model and in multiple cell types, including yeast, HEK293T, and motor-neuron-like NSC-34 cells.