Evan Z. Macosko

Jan 29, 2024 | thetransmitter.org | Joshua R. Sanes |Evan Z. Macosko

Joshua R. Sanes Professor of Molecular and Cellular Biology, Harvard UniversityContributing editor, The Transmitter Tags: Defining cell types, Cellular neuroscience, Gene expression, Neural circuits, Transcriptomics Although the brain has been an object of fascination for centuries, neurobiology as we know it originated with Santiago Ramon y Cajal’s magnificent descriptions and drawings of neurons in the late 1800s.

Dec 13, 2023 | broadinstitute.org | Sarah Williams |fei chen |Evan Z. Macosko

One cell at a timeSlide-seq, reported in 2019, involves transferring slices of tissue onto arrays of special beads, each one tagged with a DNA barcode that identifies its location in the array. The beads are 10 micrometers in diameter — about the same size as many cells in the body, so most beads only bind to the messenger RNAs from one cell. However, some beads capture the RNA of multiple cells at once. “So it meant that we don’t truly get single cell resolution with Slide-seq,” said Chen.

Jul 19, 2023 | nature.com | Seth Winfree |Qiwen Hu |Ricardo Melo Ferreira |Daria Barwinska |fei chen |Sushrut S. Waikar | +12 more

Statistics and reproducibilityFor 3D imaging and immunofluorescence staining experiments, each staining was repeated on at least two separate individuals or separate regions. For immunofluorescence validation studies, commercially available antibodies were used; 13 out of the 15 tissue samples were also analysed using snCv3 or scCv3. For ISH, 6 tissue samples (4 biopsies and 2 nephrectomies) were analysed.

Mar 12, 2023 | spectrumnews.org | Michael Fergenson |Evan Z. Macosko

Illustration by Laurène Boglio A melange of mouse research had scientists tweeting up a storm this week. It all started with “two huge spatial atlases of the whole mouse brain,” tweeted Lukas Valihrach of the Czech Academy of Sciences.