Florian A. Rosenberger

4 weeks ago | nature.com | Florian A. Rosenberger |Sophia C. Mädler |Katrine Holtz Thorhauge |Malin Fromme |Mikhail Lebedev |Marc Oeller | +7 more

AbstractProtein misfolding diseases, including α1-antitrypsin deficiency (AATD), pose substantial health challenges, with their cellular progression still poorly understood1,2,3. We use spatial proteomics by mass spectrometry and machine learning to map AATD in human liver tissue. Combining Deep Visual Proteomics (DVP) with single-cell analysis4,5, we probe intact patient biopsies to resolve molecular events during hepatocyte stress in pseudotime across fibrosis stages.

Apr 29, 2024 | nature.com | Florian A. Rosenberger |Arnaud Mourier |Diana Rubalcava-Gracia |Polyxeni Papadea |Olov Andersson |Raffaella Di Lucrezia | +6 more

AbstractThe oxidative phosphorylation system1 in mammalian mitochondria plays a key role in transducing energy from ingested nutrients2. Mitochondrial metabolism is dynamic and can be reprogrammed to support both catabolic and anabolic reactions, depending on physiological demands or disease states. Rewiring of mitochondrial metabolism is intricately linked to metabolic diseases and promotes tumour growth3,4,5.

May 10, 2023 | nature.com | Dari Shalon |Rebecca Neal Culver |Jessica A. Grembi |Handuo Shi |Florian A. Rosenberger |Sean P Spencer | +5 more

AbstractThe spatiotemporal structure of the human microbiome1,2, proteome3 and metabolome4,5 reflects and determines regional intestinal physiology and may have implications for disease6. Yet, little is known about the distribution of microorganisms, their environment and their biochemical activity in the gut because of reliance on stool samples and limited access to only some regions of the gut using endoscopy in fasting or sedated individuals7.