Francesco Simonetti

Nov 28, 2024 | cell.com | Milica Moskovljevic |Filippo Dragoni |Nathan L. Board |Fengting Wu |Jun S. Lai |Hao Zhang | +10 more

Keywords HIV-1 persistence HIV-1 latent reservoir latency reversal antigenic stimulation dendritic cells CD4+ T cells cytomegalovirus Introduction Antiretroviral therapy (ART) effectively blocks viral replication, but HIV-1 persists in a small pool of latently infected resting CD4+ T cells harboring inducible, replication-competent proviruses.1,2,3 This reservoir was originally characterized with a quantitative viral outgrowth assay (QVOA) in which resting CD4+ T cells are stimulated with...

Jan 26, 2024 | nature.com | Nathan L. Board |Milica Moskovljevic |Francesco Simonetti |Janet D. Siliciano |Luis Montaner |Sung Soo Mun

AbstractThe persistence of CD4+ T cells carrying latent human immunodeficiency virus-1 (HIV-1) proviruses is the main barrier to a cure. New therapeutics to enhance HIV-1-specific immune responses and clear infected cells will probably be necessary to achieve reduction of the latent reservoir. In the present study, we report two single-chain diabodies (scDbs) that target the HIV-1 envelope protein (Env) and the human type III Fcγ receptor (CD16).

Feb 20, 2023 | cell.com | Emily J. Fray |Fengting Wu |Francesco Simonetti |Carolin Zitzmann

With this general formula, multiple models can be tested. If we fix b3=0, then the third phase of decay starting at time Ts is flat and remains at the value of y attained at time Ts. On the other hand, if we fix Ts at a large value (say Ts=10,000) then we never reach Ts and have the usual biphasic decay model. In this latter case, with A = 0, we can test for a single decay model, estimating only b2. Note that in the case of a single decay, b1 is not estimated.